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We put the spotlight on recent work by Malaker et al., to empirically decode the mucinome.

We envisioned using APEX2 proximity tagging in a 'baitless' manner to biotinylate and identify proteins on cell surfaces. Note: We find that anchoring APEX2 on cell surfaces (via cell surface engineering) is important to get tagging to work. We also find that N-linked glycosylation occludes some protein residues for radical-mediated tagging (see SI). A fun collaboration with Naomi Kamasawa to image APEX2-labelled cell surfaces using TEM (right).

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Our editorial piece illustrating the need to study protein glycoconjugates as replete entities and the latest advancements to study their structures, interactions, and functions. 

Mia: Among the greatest joys of my career thus far has been to serve as a guest editor alongside Editor-in-Chief Prof. Laura Kiessling, for this special issue. 

companion article detailing our design, protocols, and procedures to use proximity tagging as a means of capturing GBP-glycoprotein interactions in live cells.

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 A review article discussing the various methods and applications of cell surface glycan engineering, it's history and future directions.


We present the use of in situ proximity labeling as a powerful approach to tag the dynamic glycoprotein interactors for galectin-3 in the context of living hepatic stellate cells. 

Proc Natl Acad Sci (2020) 117:27329.


 A review article surveying modern approaches towards the identification and analysis of glycan-protein intereractions. 

ACS Symposium Series (2020) 1346:1


 A conference report summarizing the discussions held at the Nanolithography of Biointerfaces Faraday Discussion in London, UK. 


A companion protocol article to accompany our work on the small molecule, surfen, which antagonizes cell surface heparan sulfate to maintain the pluripotency of mouse embryonic stem cells in culture.


25. Influencing Early Stages of Neuromuscular Junction Formation through Glycocalyx Engineering

ML Huang, EM Tota, TM Lucas, K Godula 

ACS Chem Neuro. (2018) 9:3086.

24. Embryonic Stem Cell Engineering with a Glycomimetic FGF2/BMP4 Co-Receptor Drives Mesodermal Differentiation in a Three-Dimensional Culture.

MR NaticchiaLK LaubachEM TotaTM LucasML HuangK Godula*

ACS Chem. Biol. (2018) 13:2880.

23. Glycocalyx scaffolding to control cell surface glycan displays. 

ML Huang, EM Tota, S Verespy. 

Curr Prot Chem Biol (2018) 10:e40.

22. Heparin-fibronectin interactions in the development of extracellular matrix insolubility. 

I Raitman, ML Huang, SA Williams, B Friedman, K Godula, JE Schwarzbauer. 

Matrix Biol (2018) 67:107.

21. Small molecule antagonist of cell surface glycosaminoglycans restricts embryonic stem cells in a pluripotent state. 

ML Huang, AL Michalak, CJ Fisher, M Christy, RAA Smith, K Godula.

Stem Cells (2018) 36:45.


20. Glycocalyx scaffolding with synthetic nanoscale glycomaterials.

ML Huang, S Purcell, S Verespy, Y Wang, K Godula. 

Biomat Sci (2017) 5:1537.

19. Human milk oligosaccharides inhibit growth of group B Streptococcus. 

AE Lin, CA Autran, A Szyszka, T Escajadillo, ML Huang, K Godula, AR Prudden, G-J Boons, AL Lewis, KS Doran, V Nizet, L Bode.

J Biol Chem (2017) 292:11243.

18. Synthetic mucus nanobarriers for identification of glycan-dependent primary Influenza A infection inhibitors. 

M Cohen, HP Senaati, CJ Fisher, ML Huang, P Gagneux, K Godula. 

ACS Cent Sci (2016) 2:710.

17. Capture and characterization of influenza A virus from primary samples using glycan bead arrays. 

M Cohen, CJ Fisher, ML Huang, LL Lindsay, M Plancarte, WM Boyce, K Godula, P Gagneux. 

Virology (2016) 493:128.

16.  Glycomaterials in immunology: exploring the roles of glycans integral to pathogen interactions and the accompanying host immune response.

ML Huang, CJ Fisher, K Godula. 

Exp Biol Med (2016) 241:1042.

15.  Nanoscale materials for probing the biological functions of the glycocalyx. 

ML Huang, K Godula. 

Glycobiology (2016) 26:797.

14.   Glycocalyx remodeling with glycopolymer-based proteoglycan mimetics. 

ML Huang, RA Smith, GW Trieger, K Godula. 

Methods Mol Biol (2016) 1367:207.

13.   Determination of receptor specificities for whole influenza viruses using multivalent glycan arrays

ML Huang, M Cohen, CJ Fisher, RT Schooley, P Gagneux, K Godula. 

Chem Commun (2015) 51:5326. 

12.  Priming the cellular glycocalyx for neural development. 

ML Huang, K Godula. 

ACS Chem Neurosci (2014) 5:873.

11.  Glycocalyx remodeling with proteoglycan mimetics promotes neural specification in embryonic stem cells. 

ML Huang,* RA Smith,* GW Trieger, K Godula. * equal authorship

J Am Chem Soc (2014) 136:10565. 


For prior work, click here.

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