AT SCRIPPS RESEARCH
AE Reeves and ML Huang
A concise review of chemical biology methods applied towards the study of proteoglycans.
A chemical biology-based strategy to build proteoglycans to study their function and interactions in live cells.
We envisioned using APEX2 proximity tagging in a 'baitless' manner to biotinylate and identify proteins on cell surfaces. Note: We find that anchoring APEX2 on cell surfaces (via cell surface engineering) is important to get tagging to work. We also find that N-linked glycosylation occludes some protein residues for radical-mediated tagging (see SI). A fun collaboration with Naomi Kamasawa to image APEX2-labelled cell surfaces using TEM (right).
M Critcher, AA Hassan, ML Huang*
Our editorial piece illustrating the need to study protein glycoconjugates as replete entities and the latest advancements to study their structures, interactions, and functions.
Galectin-1 is a potent activator of muscle differentiation, and it is being evaluated as a therapeutic for muscle repair. Towards identifying the molecular mechanisms through which Galectin-1 exerts its effects, we identify its glycan-mediated interactors using proximity tagging coupled with quantitative mass spectrometry.
A companion article detailing our design, protocols, and procedures to use proximity tagging as a means of capturing GBP-glycoprotein interactions in live cells.
E Joeh, A Reeves, CG Parker ML Huang*
Proc Natl Acad Sci (2020) 117:27329.
We present the use of in situ proximity labeling as a powerful approach to tag the dynamic glycoprotein interactors for galectin-3 in the context of living hepatic stellate cells.
ACS Symposium Series (2020) 1346:1
A review article surveying modern approaches towards the identification and analysis of glycan-protein intereractions.
A conference report summarizing the discussions held at the Nanolithography of Biointerfaces Faraday Discussion in London, UK.
A companion protocol article to accompany our work on the small molecule, surfen, which antagonizes cell surface heparan sulfate to maintain the pluripotency of mouse embryonic stem cells in culture.
POSTDOCTORAL WORK AT UC SAN DIEGO
ACS Chem Neuro. (2018) 9:3086.
ML Huang, EM Tota, S Verespy.
Curr Prot Chem Biol (2018) 10:e40.
I Raitman, ML Huang, SA Williams, B Friedman, K Godula, JE Schwarzbauer.
Matrix Biol (2018) 67:107.
ML Huang, AL Michalak, CJ Fisher, M Christy, RAA Smith, K Godula.
Stem Cells (2018) 36:45.
ML Huang, S Purcell, S Verespy, Y Wang, K Godula.
Biomat Sci (2017) 5:1537.
AE Lin, CA Autran, A Szyszka, T Escajadillo, ML Huang, K Godula, AR Prudden, G-J Boons, AL Lewis, KS Doran, V Nizet, L Bode.
J Biol Chem (2017) 292:11243.
M Cohen, HP Senaati, CJ Fisher, ML Huang, P Gagneux, K Godula.
ACS Cent Sci (2016) 2:710.
M Cohen, CJ Fisher, ML Huang, LL Lindsay, M Plancarte, WM Boyce, K Godula, P Gagneux.
Virology (2016) 493:128.
ML Huang, CJ Fisher, K Godula.
Exp Biol Med (2016) 241:1042.
ML Huang, K Godula.
Glycobiology (2016) 26:797.
ML Huang, RA Smith, GW Trieger, K Godula.
Methods Mol Biol (2016) 1367:207.
ML Huang, M Cohen, CJ Fisher, RT Schooley, P Gagneux, K Godula.
Chem Commun (2015) 51:5326.
ML Huang, K Godula.
ACS Chem Neurosci (2014) 5:873.
ML Huang,* RA Smith,* GW Trieger, K Godula. * equal authorship
J Am Chem Soc (2014) 136:10565.
For prior work, click here.