publications
​AT SCRIPPS RESEARCH
42. Surfaceome profiling identifies basigin-chaperoned protein clients.
39. Chemoproteomic mapping of human milk oligosaccharide (HMO) interactions in cells.
AA Hassan, JM Wozniak, Z Vilen, W Li, A Jadhav, CG Parker, ML Huang
38. Mucopedia101: Capturing and assigning mucin-domain glycoproteins.
AE Reeves and ML Huang
We put the spotlight on recent work by Malaker et al., to empirically decode the mucinome.
A concise review of chemical biology methods applied towards the study of proteoglycans.
A chemical biology-based strategy to build proteoglycans to study their function and interactions in live cells.
36. Chemical editing of proteoglycan architecture.
T O'Leary,* M Critcher,* TN Stephenson, X Yang, A Hassan, NM Bartfield, R Hawkins, ML Huang
Nat Chem Biol (2022) 18, 634-642 https://doi.org/10.1038/s41589-022-01023-5
35. Cell surface engineering enables surfaceome profiling.
We envisioned using APEX2 proximity tagging in a 'baitless' manner to biotinylate and identify proteins on cell surfaces. Note: We find that anchoring APEX2 on cell surfaces (via cell surface engineering) is important to get tagging to work. We also find that N-linked glycosylation occludes some protein residues for radical-mediated tagging (see SI). A fun collaboration with Naomi Kamasawa to image APEX2-labelled cell surfaces using TEM (right).
Mia: Among the greatest joys of my career thus far has been to serve as a guest editor alongside Editor-in-Chief Prof. Laura Kiessling, for this special issue.
33. Seeing the forest through the trees: Characterizing the glycoproteome.
M Critcher, AA Hassan, ML Huang*
Our editorial piece illustrating the need to study protein glycoconjugates as replete entities and the latest advancements to study their structures, interactions, and functions.
A companion article detailing our design, protocols, and procedures to use proximity tagging as a means of capturing GBP-glycoprotein interactions in live cells.
31. Mapping glycan to glycan-binding protein (GBP) interactions by live cell proximity tagging.
E Joeh, A Reeves, CG Parker ML Huang*
Curr Prot Chem Biol (2021) https://doi.org/10.1002/cpz1.104
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A review article discussing the various methods and applications of cell surface glycan engineering, it's history and future directions.
29. Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling.
E Joeh, T O'Leary, W Li, R Hawkins, J Hung, CG Parker, ML Huang*
Proc Natl Acad Sci (2020) 117:27329.
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We present the use of in situ proximity labeling as a powerful approach to tag the dynamic glycoprotein interactors for galectin-3 in the context of living hepatic stellate cells.
E Joeh, Z Vilen, T O'Leary, ML Huang*
ACS Symposium Series (2020) 1346:1
A review article surveying modern approaches towards the identification and analysis of glycan-protein intereractions.
A conference report summarizing the discussions held at the Nanolithography of Biointerfaces Faraday Discussion in London, UK.
S Chatterjee, TN Stephenson, AL Michalak, K Godula,* ML Huang*
Methods Enzymol. (2019) 626:249. (*co-corresponding authors)
A companion protocol article to accompany our work on the small molecule, surfen, which antagonizes cell surface heparan sulfate to maintain the pluripotency of mouse embryonic stem cells in culture.
​POSTDOCTORAL WORK AT UC SAN DIEGO​
25. Influencing Early Stages of Neuromuscular Junction Formation through Glycocalyx Engineering
ML Huang, EM Tota, TM Lucas, K Godula
ACS Chem Neuro. (2018) 9:3086.
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MR Naticchia, LK Laubach, EM Tota, TM Lucas, ML Huang, K Godula*
ACS Chem. Biol. (2018) 13:2880.
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23. Glycocalyx scaffolding to control cell surface glycan displays.
ML Huang, EM Tota, S Verespy.
Curr Prot Chem Biol (2018) 10:e40.
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22. Heparin-fibronectin interactions in the development of extracellular matrix insolubility.
I Raitman, ML Huang, SA Williams, B Friedman, K Godula, JE Schwarzbauer.
Matrix Biol (2018) 67:107.
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ML Huang, AL Michalak, CJ Fisher, M Christy, RAA Smith, K Godula.
Stem Cells (2018) 36:45.
20. Glycocalyx scaffolding with synthetic nanoscale glycomaterials.
ML Huang, S Purcell, S Verespy, Y Wang, K Godula.
Biomat Sci (2017) 5:1537.
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19. Human milk oligosaccharides inhibit growth of group B Streptococcus.
AE Lin, CA Autran, A Szyszka, T Escajadillo, ML Huang, K Godula, AR Prudden, G-J Boons, AL Lewis, KS Doran, V Nizet, L Bode.
J Biol Chem (2017) 292:11243.
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M Cohen, HP Senaati, CJ Fisher, ML Huang, P Gagneux, K Godula.
ACS Cent Sci (2016) 2:710.
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17. Capture and characterization of influenza A virus from primary samples using glycan bead arrays.
M Cohen, CJ Fisher, ML Huang, LL Lindsay, M Plancarte, WM Boyce, K Godula, P Gagneux.
Virology (2016) 493:128.
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16. Glycomaterials in immunology: exploring the roles of glycans integral to pathogen interactions and the accompanying host immune response.
ML Huang, CJ Fisher, K Godula.
Exp Biol Med (2016) 241:1042.
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15. Nanoscale materials for probing the biological functions of the glycocalyx.
ML Huang, K Godula.
Glycobiology (2016) 26:797.
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14. Glycocalyx remodeling with glycopolymer-based proteoglycan mimetics.
ML Huang, RA Smith, GW Trieger, K Godula.
Methods Mol Biol (2016) 1367:207.
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13. Determination of receptor specificities for whole influenza viruses using multivalent glycan arrays.
ML Huang, M Cohen, CJ Fisher, RT Schooley, P Gagneux, K Godula.
Chem Commun (2015) 51:5326.
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12. Priming the cellular glycocalyx for neural development.
ML Huang, K Godula.
ACS Chem Neurosci (2014) 5:873.
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ML Huang,* RA Smith,* GW Trieger, K Godula. * equal authorship
J Am Chem Soc (2014) 136:10565.
For prior work, click here.
