AT SCRIPPS RESEARCH
43. Stereoselective synthesis of photoactivatable Man(β1-4)GlcNAc-based bioorthogonal probes.
42. Surfaceome profiling identifies basigin-chaperoned protein clients.
Z Vilen, E Joeh, E Lee, and ML Huang
41. (Glycan binding) activity-based protein profiling in cells enabled by mass spectrometry-based proteomics.
Z Vilen, AE Reeves and ML Huang
40. Proximity tagging technologies to illuminate glycan-protein interactions.
39. Chemoproteomic mapping of human milk oligosaccharide (HMO) interactions in cells.
AA Hassan, JM Wozniak, Z Vilen, W Li, A Jadhav, CG Parker, ML Huang
38. Mucopedia101: Capturing and assigning mucin-domain glycoproteins.
AE Reeves and ML Huang
We put the spotlight on recent work by Malaker et al., to empirically decode the mucinome.
37. Excavating proteoglycan structure-function relationships: Modern approaches to capture the interactions of ancient biomolecules.
A concise review of chemical biology methods applied towards the study of proteoglycans.
A chemical biology-based strategy to build proteoglycans to study their function and interactions in live cells.
36. Chemical editing of proteoglycan architecture.
T O'Leary,* M Critcher,* TN Stephenson, X Yang, A Hassan, NM Bartfield, R Hawkins, ML Huang
Nat Chem Biol (2022) 18, 634-642 https://doi.org/10.1038/s41589-022-01023-5
35. Cell surface engineering enables surfaceome profiling.
Z Vilen, A Reeves, T O'Leary, E Joeh, N Kamasawa, ML Huang*
We envisioned using APEX2 proximity tagging in a 'baitless' manner to biotinylate and identify proteins on cell surfaces. Note: We find that anchoring APEX2 on cell surfaces (via cell surface engineering) is important to get tagging to work. We also find that N-linked glycosylation occludes some protein residues for radical-mediated tagging (see SI). A fun collaboration with Naomi Kamasawa to image APEX2-labelled cell surfaces using TEM (right).
Mia: Among the greatest joys of my career thus far has been to serve as a guest editor alongside Editor-in-Chief Prof. Laura Kiessling, for this special issue.
33. Seeing the forest through the trees: Characterizing the glycoproteome.
M Critcher, AA Hassan, ML Huang*
Our editorial piece illustrating the need to study protein glycoconjugates as replete entities and the latest advancements to study their structures, interactions, and functions.
32. Proximity tagging identifies the glycan-mediated glycoprotein interactors of galectin-1 in muscle stem cells.
Z Vilen, E Joeh, M Critcher, CG Parker ML Huang*
Galectin-1 is a potent activator of muscle differentiation, and it is being evaluated as a therapeutic for muscle repair. Towards identifying the molecular mechanisms through which Galectin-1 exerts its effects, we identify its glycan-mediated interactors using proximity tagging coupled with quantitative mass spectrometry.
A companion article detailing our design, protocols, and procedures to use proximity tagging as a means of capturing GBP-glycoprotein interactions in live cells.
31. Mapping glycan to glycan-binding protein (GBP) interactions by live cell proximity tagging.
E Joeh, A Reeves, CG Parker ML Huang*
Curr Prot Chem Biol (2021) https://doi.org/10.1002/cpz1.104
30. Glycoengineering: Scratching the Surface.
M Critcher, T O'Leary, ML Huang*
A review article discussing the various methods and applications of cell surface glycan engineering, it's history and future directions.
29. Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling.
E Joeh, T O'Leary, W Li, R Hawkins, J Hung, CG Parker, ML Huang*
Proc Natl Acad Sci (2020) 117:27329.
We present the use of in situ proximity labeling as a powerful approach to tag the dynamic glycoprotein interactors for galectin-3 in the context of living hepatic stellate cells.
28. Recent advancements in arrayed technologies and emerging themes in the identification of glycan-protein interactions
E Joeh, Z Vilen, T O'Leary, ML Huang*
ACS Symposium Series (2020) 1346:1
A review article surveying modern approaches towards the identification and analysis of glycan-protein intereractions.
27. Highlights from Faraday Discussion 301: Nanolithography of Biointerfaces (London, UK, July 3-5, 2019)
CS Mahon,* ML Huang* (*co-corresponding authors)
A conference report summarizing the discussions held at the Nanolithography of Biointerfaces Faraday Discussion in London, UK.
26. Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonists.
S Chatterjee, TN Stephenson, AL Michalak, K Godula,* ML Huang*
Methods Enzymol. (2019) 626:249. (*co-corresponding authors)
A companion protocol article to accompany our work on the small molecule, surfen, which antagonizes cell surface heparan sulfate to maintain the pluripotency of mouse embryonic stem cells in culture.
POSTDOCTORAL WORK AT UC SAN DIEGO
25. Influencing Early Stages of Neuromuscular Junction Formation through Glycocalyx Engineering
ML Huang, EM Tota, TM Lucas, K Godula
ACS Chem Neuro. (2018) 9:3086.
24. Embryonic Stem Cell Engineering with a Glycomimetic FGF2/BMP4 Co-Receptor Drives Mesodermal Differentiation in a Three-Dimensional Culture.
MR Naticchia, LK Laubach, EM Tota, TM Lucas, ML Huang, K Godula*
ACS Chem. Biol. (2018) 13:2880.
23. Glycocalyx scaffolding to control cell surface glycan displays.
ML Huang, EM Tota, S Verespy.
Curr Prot Chem Biol (2018) 10:e40.
22. Heparin-fibronectin interactions in the development of extracellular matrix insolubility.
I Raitman, ML Huang, SA Williams, B Friedman, K Godula, JE Schwarzbauer.
Matrix Biol (2018) 67:107.
21. Small molecule antagonist of cell surface glycosaminoglycans restricts embryonic stem cells in a pluripotent state.
ML Huang, AL Michalak, CJ Fisher, M Christy, RAA Smith, K Godula.
Stem Cells (2018) 36:45.
20. Glycocalyx scaffolding with synthetic nanoscale glycomaterials.
ML Huang, S Purcell, S Verespy, Y Wang, K Godula.
Biomat Sci (2017) 5:1537.
19. Human milk oligosaccharides inhibit growth of group B Streptococcus.
AE Lin, CA Autran, A Szyszka, T Escajadillo, ML Huang, K Godula, AR Prudden, G-J Boons, AL Lewis, KS Doran, V Nizet, L Bode.
J Biol Chem (2017) 292:11243.
18. Synthetic mucus nanobarriers for identification of glycan-dependent primary Influenza A infection inhibitors.
M Cohen, HP Senaati, CJ Fisher, ML Huang, P Gagneux, K Godula.
ACS Cent Sci (2016) 2:710.
17. Capture and characterization of influenza A virus from primary samples using glycan bead arrays.
M Cohen, CJ Fisher, ML Huang, LL Lindsay, M Plancarte, WM Boyce, K Godula, P Gagneux.
Virology (2016) 493:128.
16. Glycomaterials in immunology: exploring the roles of glycans integral to pathogen interactions and the accompanying host immune response.
ML Huang, CJ Fisher, K Godula.
Exp Biol Med (2016) 241:1042.
15. Nanoscale materials for probing the biological functions of the glycocalyx.
ML Huang, K Godula.
Glycobiology (2016) 26:797.
14. Glycocalyx remodeling with glycopolymer-based proteoglycan mimetics.
ML Huang, RA Smith, GW Trieger, K Godula.
Methods Mol Biol (2016) 1367:207.
13. Determination of receptor specificities for whole influenza viruses using multivalent glycan arrays.
ML Huang, M Cohen, CJ Fisher, RT Schooley, P Gagneux, K Godula.
Chem Commun (2015) 51:5326.
12. Priming the cellular glycocalyx for neural development.
ML Huang, K Godula.
ACS Chem Neurosci (2014) 5:873.
11. Glycocalyx remodeling with proteoglycan mimetics promotes neural specification in embryonic stem cells.
ML Huang,* RA Smith,* GW Trieger, K Godula. * equal authorship
J Am Chem Soc (2014) 136:10565.
For prior work, click here.